汞胁迫对大豆幼苗SOD和CAT活性的影响

摘要

采用营养液培养方法,以不同Hg2+浓度处理大豆,测定大豆种子萌发情况及其幼苗中超氧化歧化酶(SOD)和过氧化氢酶(CAT)活性.结果表明:在不同Hg2+浓度范围(0 ～200.0 mg/L)内,随着Hg2+浓度不断升高,大豆种子发芽率逐渐下降,在不含Hg2+溶液内,大豆种子发芽率最高达到100％,Hg2+浓度在200.0 mg/L时大豆种子萌发率为0;在Hg2+浓度胁迫下,低Hg2+浓度胁迫促使植株生长率上升,而随着Hg2+浓度升高,大豆植株的生长率逐渐下降,大豆植株生长受到抑制;当胁迫浓度增加,超氧化物歧化酶(SOD)和过氧化氢酶(CAT)呈先上升后下降的趋势;Hg2+对大豆幼苗生长抑制程度与处理浓度呈正相关性.%A hydroponic experiment was conducted to study the effects of using different Hg2+ concentration on soybean seedlings growth and its determination seeding super oxide dismutase (SOD) and catalase (CAT) activity.The results showed that the activity change of soybean seed germination rate,plant height,superoxide dismutase (SOD) and catalase (CAT) in different concentration range (0-200.0 mg/L).soybean seed germination rate decreased with the rise of Hg2+ concentration.While excluding Hg2+,the highest rate of soybean seed germination,reaching 100％.In Hg2+ concentration is 200.0 mg/L,seed germination rate was 0％.Under the stress of low Hg2+ concentration plant growth rate,as Hg2+ concentration increased plant growth is restrained.But with the increase of stress strength,superoxide dismutase (SOD) and catalase (CAT) present a tendency of falling after rising first.Hg2+ concentration inhibitory concentration degree and processing of soybean seedlings into positive correlation.