毛白杨纤维素合酶基因家族部分成员的克隆及表达

摘要

Wood is an attractive renewable source of lignocelluiosic biomass for biofuels production and pulping industry. One of the principal components of wood is cellulose, determining the morphology of plants and wood quality. In this study, we isolate seven cellulose synthase genes: PtoCesA4,PtoCesA5,PtoCesA7,PtoCesA8,PtoCesA13,PtoCesA17 and PtoCesA18 from Populus tomentosa by using RT-PCR and bioinformatics methods. A phylogenetic tree of Arabidopsis thaliana, Oryza sativa, Populus trichocarpa and P. Tomentosa cellulose synthase gene superfamilies was constructed using the MEGA4. 0 program. GenomeLabTM GeXP genetic analysis showed that the transcription levels of PtoCesAll and PtoCesAl along with PtoCesA4,PtoCesA8 and PtoCesA18 were much higher in xylem than that in leaves in P. Tomentosa, implying that these genes may function on secondary wall formation.%从毛白杨中分离7个纤维素合酶基因,分别为PtoCesA4,PtoCesA5,PtoCesA7,PtoCesA8,PtoCesA13,Pto CesA 17和PtoCesA18,通过与已公布的毛果杨全基因序列及拟南芥和水稻基因组中纤维素合酶基因序列的同源性比对分析,结合目前纤维素合成机制的研究进展,预测毛白杨中上述不同纤维素合酶的功能.利用GenomeLabTMGeXP遗传分析系统分析毛白杨中纤维素合酶基因在不同组织中的转录表达水平,结果表明AoCesA4,PtoCesA7,PtoCesA8,PtoCesA17和PtoCesA18在木质部高丰度表达,推测这些基因可能参与毛白杨次生细胞壁的形成,为毛白杨次生细胞壁中纤维素的合成调控提供参考依据.