An entomopathogenic fungal strain isolated from infected Melanotus cribricollis larvae was identified as Metarhizium pingshaense by both morphological and molecular methods. The 5' end of EF-1α of the isolate was sequenced, and submitted to GenBank with accession number JQ081264. Study on biological characteristics of the isolate showed that the optimum culture medium was PPDA. The range of optimum temperature for mycelium growth and conidia germination was from 25 ℃ to 30 ℃ , respectively and the optimum temperature for conidia production was 25 ℃. The most suitable pH for conidia germination was 7. 0. Conidia germination of this isolate could be obviously inhibited by ultraviolet radiation for less than 30 minutes.
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