绿盲蝽在不同棉花品种(系)上取食行为的EPG解析及田间验证

摘要

[Objective]The objective of this study is to observe and analyze the feeding behaviors of lygus bugs (Apolygus lucorum Meyer-Dür) on different cotton varieties and to understand the feeding mechanism of A.lucorum, so that the cotton germplasm with resistance to lygus bugs can be bred.[Method]Electrical penetration graph device (Giga-8 DC-EPG) and stereomicroscope were conducted to observe the feeding behaviors of A.lucorum on 14 different cotton varieties (lines), and the occurrence and damage dynamics of A.lucorum population were investigated in the cotton field.[Result]The results show that there are five types of sucking waveforms produced by A.lucorum including A wave, H wave, M wave, B wave, and non-probing wave (NP wave), respectively.A wave is a spying wave, H wave is a stylet probing in the phloem wave, M wave is a stylet probing into the phloem to break cells and secret saliva wave, B wave is a chopping cells mixture and stylet pulling after staying wave, NP wave is a beak and the stylet staying on the leaf surface without piercing wave.[Conclusion]Asian cotton and GK50 have a strong repellence to A.lucorum.However, A.lucorum showed great preference to varieties Ling-06 and Shikang 338.It is concluded that indoor analytical results are consistent with the investigation of the occurrence and damage of A.lucorum in cotton field.%[目的]观察并解析绿盲蝽在不同棉花品种(系)植株上的取食选择行为,阐明绿盲蝽取食危害机制,为发掘抗盲蝽棉花种质资源提供依据.[方法]采用直流昆虫刺探电位仪Giga-8 DC-EPG,结合体视显微镜装置观察绿盲蝽对14个不同品种(系)棉花植株的取食行为,同时田间调查不同棉花品种(系)上绿盲蝽种群发生危害动态并确定相应的绿盲蝽危害级别.[结果]绿盲蝽的刺探波型主要包括5种波型,即A波,H波、M波、B波,以及非刺探波型NP波.A波为刺探波,H波为口针在韧皮部刺探波,M波为口针进入韧皮部中破碎细胞并分泌唾液波,B波为对搅碎细胞后混合物的吸取以及口针停留后拔出波,NP波为喙和口针停留在叶片表面并未刺入波.[结论]14个棉花品种(系)中,亚洲棉和GK50对绿盲蝽表现较强的驱避性,而绿盲蝽对灵-06、石抗338等棉花品种(系)有较强的趋向性.室内解析与田间调查绿盲蝽种群发生危害动态及危害级别结果一致.