[目的]证明纤维肉瘤病料中含有的ALV-J相关病毒能够诱发急性纤维肉瘤.[方法]将含ALV-J相关病毒的病料过滤液经不同部位接种1日龄817肉杂鸡;将该过滤液不同倍数稀释后接种1日龄SPF鸡和817肉杂鸡;将该过滤液接种细胞培养后的上清液接种1日龄817肉杂鸡,连续动态观测30d.[结果]颈部皮下、胸肌、腹腔3个部位攻毒鸡的肿瘤发生率分别为100％ (20/20)、95.2％ (20/21)、100％ (20/20).病料过滤液未经稀释接种817肉杂鸡和SPF鸡的肿瘤发生率均为100％ (10/10),10倍稀释后攻毒鸡的肿瘤发生率分别为80％( 8/10)和100％ (10/10),1 00倍稀释后攻毒鸡的肿瘤发生率分别为100％ (10/10)和80％( 8/10),1000倍稀释后攻毒鸡的肿瘤发生率分别为40％( 4/10)和50％( 5/10),更高倍数稀释后攻毒鸡没有肿瘤发生.接种病料的细胞培养上清也能使33.3％ (2/6)的鸡只发生肉瘤.病理组织学检测显示:诱发的急性肿瘤系典型的纤维肉瘤.[结论]817肉杂鸡肉瘤病料中含有ALV-J相关的急性致肿瘤病毒,不论是病料过滤液本身还是其接种DF-1细胞培养后的上清液都能在不同品种鸡复制出急性纤维肉瘤,最快在接种后7d出现,且肿瘤发生的时间、动态和肿瘤发生率与接种量密切相关.%[Objective] An experiment was carried out to prove that the field chicken fibrosarcoma mass contains acute fibrosarcoma-inducing ALV-J associated virus. [Method] One-day-old 817 hybrid chickens were inoculated through different routes with tumor suspension filtrate containing ALV-J associated virus. One-day-old 817 hybrid chickens and SPF Leghan chickens were inoculated subcutaneously with tumor suspension filtrate containing ALV-J associated virus in different doses. Continuous observation of tumor development was made in 30 days. [Result] In the groups of inoculations through the subcutaneous, in muscule and intra-abdominal, the tumor incidence was 100% (20/20), 95.2% (20/21), and 100% (20/20), respectively. The tumor incidence of 817 hybrids and SPF chickens inoculated with undiluted tumor extract filtrate was 100% (10/10). When birds were inoculated with tumor extracts in 1 : 10 and 1 : 100, tumor rates were still in the range of 80% (8/10) to 100% (10/10) for both chicken lines. When birds were inoculated with tumor extracts in 1 : 1 000, tumor rates decreased to 40% (4/10) or 50% (5/10). No tumor development was observed in 30 days when birds were inoculated with the tumor extract of 1 : 10 000 dilution. However, there were 2/6 chickens developed tumor mass when inoculated with supernatant from tumor extract-infected DF-1 cell culture. Histopathology indicated that the induced tumors were typical fibrosarcoma. [Conclusion] The fibrosarcoma tissues of 817 hybrid chickens of field case contained ALV-J associated acutely tumorigenic virus. Both tumor extract filtrate or extract-infected DF-1 cell culture supernatant could induce acute fibrosarcomas in chickens of different lines, and the tumor started to appear as early as 7 days after inoculation. The dynamics of the fibrosarcoma development and tumor rates were closely related to doses of the tumor extracts in inoculation.
展开▼
