Static experiments were designed to investigate the process and influencing factors of nitrobenzene degradation coupled to bacterial dissimilatory iron reduction in subsurface environment.The result indicated that iron reducing bacterial could utilize simple organics as electron donor to reduce goethite.With the rise of the concentration of nitrobenzene which could be used as the sole electron donor,the growth of bacterial was restrained gradually and stopped while its concentration over 600 mg/L.The highest removal efficiency of nitrobenzene was observed at 0.3 mg/L goethite and 2 × 108 Cells/mL microbial biomass system,and the degradation rate of nitrobenzene was over 78.5％.Electron shuttle,humic acid,could enhance the attenuation of nitrobenzene,and its degradation rate was up to 88.8％.However,VB2 inhibites the degradation of nitrobenzene.%通过静态实验对地下环境中微生物异化还原降解硝基苯作用及其影响因素进行了研究.结果表明,铁还原微生物能以简单有机物为碳源生长并还原针铁矿,硝基苯可以作为唯一碳源被铁还原微生物利用.随着硝基苯浓度的升高,铁还原微生物的生长逐渐受到抑制.硝基苯浓度为600 mg/L时,铁还原微生物的生长停滞.针铁矿浓度0.3 mg/L、铁还原微生物浓度2×108 cells/mL时耦合体系对硝基苯降解效果最好,硝基苯降解率达78.5％以上.腐植酸能作为电子穿梭体促进耦合体系中硝基苯的衰减,硝基苯降解率达88.8％以上,而维生素B2(VB2)抑制了硝基苯的降解.
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