GGR6浸种处理浓度对沙打旺种子活力及萌发特性的影响

摘要

利用植物生长调节剂调节植物生长、发育和繁殖,是有效提高植物对逆境适应能力和生产能力的重要手段.为探究植物生长调节剂GGR6对沙打旺种子活力及萌发特性的影响,通过观测不同GGR6浓度(0、50、100、150和200 mg/L)浸种处理下,沙打旺种子活力及萌发特性指标,利用隶属函数法,综合分析筛选出适宜沙打旺种子萌发的GGR6处理浓度.结果表明:1)种子发芽动态呈“单峰型”,GGR6处理提高了种子发芽速度和发芽率,缩短发芽时间;2)处理浓度为100 mg/L以下时,种子活力、发芽势、发芽率、发芽指数、萌发指数和活力指数均随着GGR6浓度升高而升高,且浓度在50 ～ 100 mg/L之间,升高最为显著,高于100 mg/L后,各处理之间差异不显著(P＞0.05);3)促进沙打旺种子萌发的最佳GGR6作用浓度为100 mg/L.研究对利用植物措施在荒漠化土地治理中,加速植被建设的进程和效果具有重要意义.%[Background] Regulator for growth,development and reproduction of the plant,is an important means to improve adaptability and production capacity,and has a key impact on ecological construction of desert land management using plant measures.The seed germination ability affects directly the growth,reproduction and production of plants,determines the success of some plants settled in the adversity,and has a critical effect on the life history,population growth,the formation and succession of plants.Astragalus adsurgens is one of the main species for soil and water conservation and ecological construction in the Loess Plateau of China.Understanding the effects of plant growth regulators on seed vigor and germination characteristics of A.adsurgens is for improving adaptive ability to adverse environment and for increasing vegetative coverage during restoration and reconstruction of vegetation.[Methods] The concentrations of0,50,100,150 and 200 mg/L plant growth regulator GGR6 solution,respectively at 20 ℃ (G50,G100,G150,and G200) and dark conditions (CK) were designed to soak A.adsurgens seed for 24 hours,and the influences of GGR6 on seed vigor and germination characteristics of A.adsurgens were observed.Seed viability test (a constant temperature of 30 ℃) and germination test (constant temperature,relative humidity of 80％,12 h light and 12 h darkness alternating) were conducted in the artificial climate chamber for the observation of seed vigor,germination energy,germination rate,germination index and vigor index with five replications of each treatment.Excel and SAS 9.0 statistical software were used for data processing,mapping and variance analysis.The suitable GGR6 concentration for A.adsurgens seed germination was selected by membership function method.[Results] 1) The seed germination of A.adsurgens was “ unimodal type”,and the same tendency presented in the different treatments.The order of seed germination rate was CK ＞ G50 ＞ G100 ＞ G150 ＞ G200 in the first day,and peak value of germination rate (2 to 4 days) was CK ＜ G50 ＜ G100 ＜ G150 ＜ G200.2) Seed vigor,germination energy,germination rate,germination index,and vigor index of A.adsurgens increased with increasing concentration of GGR6 when GGR6 concentration was below 100 mg/ L.The effects of the treatments on seed vigor,germination energy,germination rate,and vigor index were not obvious when the GGR6 concentration was higher than that of 100 mg/L,and germination index also decreased.[Conclusions] 1) GGR6 solution improved the speed and rate of seed germination of A.adsurgens (Germination process advanced 1 day).2) Each value increased significantly (P ＜ 0.05)when the concentration were 50-100 mg/L.3) The order of seed vigor and germination characteristic indexes based on the average values of membership function was G100 ＞ G200 ＞ G150 ＞ G50 ＞ CK.The optimal GGR6 concentration was 100 mg/L for seed germination of A.adsurgens.