牛支原体感染家兔试验

摘要

To inquiry whether the rabbit was susceptible animal of Mycoplasma bovis ,this study used dif-ferent concentration gradient Mycoplasma bovis isolate through nasal drip infection in rabbits and observed infection situation by the clinical symptoms,pathological lesions,histopathological lesions in the lungs of rabbits.To ensure whether it is successfully infected,the pathogen from lungs and blood of rabbits was i-solated.After purification,it was detected by PCR and sequenc analysis.The results showed that the Myco-plasma bovis of 1.0×108 CCU/mL concentration can induce the rabbit pathogenesis,the Mycoplasma bovis of 1.0×1010 CCU/mL concentration can induce the rabbits to appear slightly spirit depression.By necrop-sy,we can find the hydrothorax,slightly marble lesions,edema and nodules in lungs.We can found alveolar hemorrhage,alveolar gap broadening,epithelial necrosis and exfoliation in mucosa of bronchioles by his-topathological lesion examination in lungs.Through the specific PCR and sequencing detection,the results verified that the pathogen,isolated from the lungs and blood of infected rabbits,was Mycoplasma bovis ,and the sera of infection rabbits kept high antibody levels.Finally,it confirmed the rabbit was susceptible ani-mal of Mycoplasma bovis .The results provide experimental basis for the establishment of animal model and further study of Mycoplasma bovis .%为了探究牛支原体（Mycoplasma bovis ）是否可以感染家兔，将不同浓度梯度的 M．bovis 分离株通过滴鼻法感染家兔呼吸道，观察感染后家兔的临床症状、剖检病理变化和肺脏组织病理变化等来确定家兔的感染情况，并从病变家兔的肺脏和血液中分离出病原，纯化后进行聚合酶链式反应（PCR）鉴定，进一步测序做亲缘关系的分析，血清抗体检测。结果表明，1．0×108 CCU／mL 的 M．bovis 能引起家兔发病，1．0×1010 CCU／mL 的 M．bovis 感染后家兔出现轻微的精神状态；剖检可见胸腔积水、肺脏轻微的大理石样病变且伴随水肿、结节等；从肺脏病变组织切片可见肺泡腔出血，肺泡间隙增宽，细支气管上皮黏膜坏死和脱落；将家兔血液和肺脏分离到的病原菌经 PCR 鉴定和测序，结果鉴定为 M．bovis，且具有较高的抗体水平，证实家兔为 M．bovis 的易感动物，为 M．bovis 动物模型的建立及 M ．bovis 的深入研究提供试验基础。