Objective: To prepare nolatrexed dihydrochloride liposmes (NDL) and detect their correlative parameters. Methods: The nolatrexed dihydrochloride liposomes were prepared by film dispersion-extrusion combined with the ammonium sulphate gradients method. The UV detector with Sephadex G50 was utilized for the determination of the drug entrapping efficiency of liposomal nolatrexed dihydrochloride. The disposition of particle diameter was investigated by transmission electron microscope(TEM) and laser particle analyzer And the entrapment efficiency and characteristic of drug release in vitro were detected by UV method. Results: The mean diameter of the nolatrexed dihydrochloride liposomes was 103.5±26 nm, with the entrapping efficiency of 83.6 ％± 2.37％. From the drug release experiment, about 66.5％ nolatrexed dihydrochloride was released from the lipidosome after 24 hours. Conclusion: The new prepared liposomes have good characteristics both in distribution and size, and it also can reach the purpose of slow-release effectiveness in-vitro,but the entrapping efficiency percentage still need to be further lifted.%目的:制备盐酸洛拉曲克脂质体并考察其理化特性.方法:采用薄膜挤压-硫酸铵梯度法制备盐酸洛拉曲克脂质体,透射电镜及激光粒度分析仪分别观察和检测其粒径大小及分布,通过紫外分光光度法测定包封率及评估体外释药试验.结果:制备的盐酸洛拉曲克脂质体包封率达83.6%±2.37%,粒径103.5±26nm且分布均匀.24h体外释放实验结果提示约有66.5%的盐酸洛拉曲克从脂质体释放出来.结论:新制备的盐酸洛拉曲克脂质体粒径大小均匀,包封率尚有提高空间,具有体外缓慢释药的特性.
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