将黄芪、黄芩、杜仲和甘草按照4∶3∶2∶1的质量比制成一定浓度的复方药液并注射锯缘青蟹,分析血淋巴中的酚氧化酶、碱性磷酸酶、酸性磷酸酶、过氧化物酶、超氧化物歧化酶、溶菌酶等相关的非特异免疫因子活力.结果表明,青蟹血清酚氧化酶、碱性磷酸酶、酸性磷酸酶、过氧化物酶、超氧化物歧化酶、溶菌酶的活力和血清抗菌活力比对照组显著提高(P＜0.05),除超氧化物歧化酶外,各种免疫酶活力在注射后先缓慢上升,2d达到最高值,然后缓慢下降,至第4天基本回复到正常水平.用副溶血弧菌攻毒7d后,注射复方中药提取液的锯缘青蟹,其免疫保护率达到53.33％.%Experiments were carried out to explore the function of Chinese herbal medicine as immunostimulants in Scylla serrata. The formula included Astragalus membranaceus, Ba-icalensis Georgi , Eucommia ulmoides Oliver, and GLycyrrhiza uralensis Fisch in the proportion of 4 : 3 : 2 : 1. The control group was injected with saline, while the experimental groups were injected with liquid formulated Chinese herbal formula medicine. The activity of enzymes in hemolymph firstly increased until the peak appeared 2-day after injection (except SOD) , then decreased back to normal level 4-day after injection. The survival rate of treatment groups was improved to 53. 33% when S. serrata was subjected to Vibrio parahaemolyticus. It is suggested that this herbal formula may be used as a herbal feed additive and serve as an effective im-munostimulant in cultured aquatic animals.
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