苜蓿褐斑病菌离体叶片接种方法及侵染途径观察

摘要

Scanning electron microscope was used to compare five indoor inoculation methods on Pseudopeziza rnedicaginis and observe the way that P .medicaginis ascospore invasion in alfalfa leaves. The results showed that the reverse spore culture technique is ideal way for P. medicaginis inoculation in comparison with the others. Spore inoculated with this method was close to natural condition of spore release. Ascospores inoculated to alfafa leaves produced germ tubes in 4 h, germ tube direct penetrated any part of alfafa leaves except pores. No infection structures like appressorium was found.%筛选比较了5种苜蓿褐斑病菌离体叶片接种技术，结果表明，逆向产孢培养技术是苜蓿褐斑病的理想接种方法。利用扫描电镜，对逆向产孢培养技术离体接种的苜蓿假盘菌（Pseudopeziza medicaginis）子囊孢子侵入途径观察发现：接种到叶片上的子囊孢子4h后开始萌发产生芽管以直接侵入的方式侵入寄主表皮细胞，侵入前不形成附着胞等侵染结构，未观察到子囊孢子由气孔侵入。