内生真菌侵染对盐胁迫下黑麦草种子萌发的影响

摘要

以低内生真菌侵染率和高内生真菌侵染率的多年生黑麦草（Lolium perenne）种子为材料,研究了在不同浓度盐胁迫下内生真菌侵染对种子萌发的影响。结果表明,高内生真菌侵染率和低内生真菌侵染率种子的相对发芽率、相对活力指数、相对根长、相对苗长、相对根苗比均随盐胁迫程度的增加而显著下降。中、低浓度盐胁迫下（≤170mmol.L-1 NaCl）二者的相对发芽率、相对活力指数、相对根长、相对苗长的差异不显著（P〉0.05）;高浓度盐胁迫下（255mmol.L-1 NaCl）,高内生真菌侵染率种子的相对发芽率、相对活力指数、相对苗长均显著大于低内生真菌侵染率的品种（P〈0.05）。盐胁迫抑制了多年生黑麦草种子的萌发,但在高浓度盐胁迫下内生真菌对宿主具有增益作用。%To evaluate the effect of salinity and endophytic fungi on seed germination of Lolium perenne, 8 perennial ryegrass varieties with high endophyte infection rate （≥80%） and 8 varieties with low rate （≤20%） were used for germination experiment under salinity stress. The results of this study showed that the relative germination rate （RGR）, relative vitality index （RVI）, relative root length （RRL）, relative shoot length （RSL） and relative root/shoot ratio （RRS） decreased with increasing NaCl concentrations. There was no significant difference observed in RGR, RVI, RRL and RSL between high and low infection rate under 0, 85, and 170 mmol·L^-1 NaCl stress, while RGR, RVI, and RSL of seeds with high infection rate were significantly （P〈0.05） higher than that of low infection rate under 255 mmol·L^-1 NaCl stress. In a word, seed germination of perennial ryegrass was inhibited by salt stress, but endophyte infection could benefit seed germination under high salinity condition.