胃痛消痞方对肝郁脾虚型FD大鼠胃泌素和生长抑素的影响

摘要

Objective To explore the influence of weitongxiaopi decoction( WTXP )on gastrin( GAS )and so-matostatin( SS )in serum, gastric and duodenal of rats with functional dyspepsia( FD )of liver-depression and spleen-deficiency type. Methods 40 Wistar rats were randomly divided into control, model, WTXP and cisapride( CP )groups. Except for control group,a rat model of functional dyspepsia was established by the improved tail clamp method. The FD rats were given WTXP or cisapride( CP )for 3 weeks. After treatment, the GAS serum in gastric and duodenal were examined by enzyme-linked immunosorbent assay( ELISA )and immuno-histochemical methods respectively. Results Compared with control group, the GAS content in model group was significantly lower( P < 0. 05 ). After treatment, compared with model group, the GAS content of WTXP group and CP group were significantly higher( P < 0. 05 ), WTXP group was significantly higher than CP group( P < 0. 05 ); each treatment group was lower than control group( P <0. 05 ). Compared with control group,the SS content of model group was significantly higher( P <0. 05 ). After treatment, SS content of WTXP group and CP group were significantly lower than that of model group( P <0. 05 );WTXP group was significantly lower than CP group;each treatment group was higher than control group( P <0. 05 ). Conclusion FD can cause the changes of the GAS and SS contents in rats. WTXP can increase the contents of GAS in serum, gastric and duodenal and decrease the content of SS in FD rats.%目的 探讨胃痛消痞方对功能性消化不良(Functional dyspepsia,FD)大鼠血清及胃窦、十二指肠组织中胃泌素(GAS)和生长抑素(SS)的影响.方法 40只Wistar大鼠随机分为正常对照组、模型组、胃痛消痞方组、西沙必利组.除正常对照组大鼠外,通过改良夹尾刺激法建立FD大鼠模型,胃痛消痞方(中药)组与西沙必利(西药)组灌服相应药物3周,模型组与对照组进行对照观察.ELISA、免疫组化测定大鼠血清与胃窦、十二指肠组织中GAS、SS含量.结果 与对照组相比,模型组大鼠GAS血清含量及胃肠组织表达减低;经中西药干预治疗后,与模型组相比,GAS含量均明显增高.中药组GAS含量显著高于西药组,差异有统计学意义(P<0.05);各治疗组GAS均低于正常对照组(P<0.05).与对照组比较,模型组大鼠SS血清含量及胃肠组织表达显著增高;经中西药干预后,与模型组相比,SS明显下降,中药组SS含量显著低于西药组,差异有统计学意义(P<0.05);各治疗组SS含量均高于正常对照组(P<0.05).结论 功能性消化不良可以引起大鼠GAS、SS水平改变,胃痛消痞方可以提高FD大鼠血清及胃窦、十二指肠组织中GAS含量,降低SS含量.