目的 观察血必净注射液对急性百草枯中毒大鼠IL-1、IL-6、TNF-α的影响,探讨血必净注射液对百草枯中毒炎症反应是否有抑制作用.方法 将30只雄性Wistar大鼠随机分为Ⅰ组(空白对照组)、Ⅱ组(百草枯染毒组)、Ⅲ组(血必净治疗组),每组10只.对照组大鼠给予生理盐水10 mL/kg灌胃,其余两组分别给予百草枯20 mg/kg灌胃染毒,染毒后30 min开始,Ⅰ、Ⅱ组大鼠给予生理盐水10 mL/kg,1次/d腹腔注射,直至处死;Ⅲ组大鼠给予血必净10 mL/kg,1次/d腹腔注射,直至处死.每日观察大鼠的生命状态,是否有死亡.分别于3、7、14 d采集大鼠内眦静脉血1.5 mL,高速离心,取上层血清,用酶联免疫吸附法检测细胞白介素1(IL-1),细胞白介素6(IL-6),肿瘤坏死因子-α(TNF-α)的表达水平,分析三组实验数据,探讨三组数值之间的相关性.结果 ①染毒组、血必净治疗组IL-1、IL-6、TNF-α表达水平较空白组明显升高,差异有统计学意义(P<0.001);②血必净治疗组IL-1、IL-6、TNF-α表达水平较染毒组明显降低,差异有统计学意义(P<0.001).结论 在急性百草枯中毒时,引起体内炎性因子增加,导致多脏器功能受损,而血必净注射液有效降低了内毒素及过量炎性介质的损伤,抑制IL-1、IL-6、TNF-α的升高,疗效较明显,为临床治疗提供了有力的证据.%Objective To observe the influence of xuebijing injection on IL-1 , IL-6,TNF-a in acute paraquat rats,and discuss whether xuebijing injection can inhibit the inflammatory response cased by paraquat poisoning. Meth-ods 30 male Wister rats were randomly divided into control group, poisoning control group, xuebijing treatment group, 10 rats each group. Control group was given saline( 10 ml,/kg I. P. ); poisoning control group and xuebijing treatment group were given paraquat( 20 mg/kg I. P. ). After 30 minutes, control group and poisoning control group were given saline( 10 mL/kg )once a day by intraperitoneal injection,xuebijing treatment group was given xuebijing( 10 mL/kg )once a day by intraperitoneal injection. To collect 1. 5 mL blood from inner and canthal vein on the 3rd day, 7th day, 14th day. To separate serum and detect the expression level of IL-1, IL-6, TNF-a and analyze the experimental datas and discuss the correlation of the three groups. Results The expression levels of IL-1,IL-6,TNF-a of poisoning control group and xuebijing treatment group were obviously higher than those of control group( P < 0. 001 ). The expression levels of IL-1,IL-6,TNF-a of xuebijing treatment group were lower than those of poisoning control group( P <0. 001 ). Conclusion Inflammatory factors increase in paraquat poisoning,which can cause multiple organ failures, but xuebijing can reduce the injury caused by inflammatory mediators and inhibit the increasing of IL-1 ,IL-6 and TNF-a. Which provide a basis for future clinical therapy.
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