高氧环境下ROS对肠上皮细胞表达TNF-α和HIF-1-α的影响

摘要

目的 探讨长时间高氧治疗对机体造成严重不良反应的原因.方法 用不同浓度的H2 O2(100、200、400μM)和85%的高氧对肠上皮细胞干预24 h后,采用免疫组织化学方法检测高氧对TNF-α和HIF-1-α的影响.结果 与对照组相比,高氧组和各H2 O2组TNF-α和HIF-1-α表达水平显著增加(P<0.05),并且高氧组明显高于H2 O2组.结论 高氧环境中肠上皮细胞的损伤伴随着ROS的增加,因此认为在高氧环境中ROS对肠道损伤发挥着重要作用.%Objective To explore the reason of severe adverse effects by prolonged hyperoxia treatment. Methods The intestinal epithelium cells were treated with different concentrations of H2O2(100,200,400 μM)and 85% oxygen for 24 h. The expression levels of TNF-αand HIF-1-αwere detected by immunohistochemistry. Results Compared with control group,the expression levels of TNF-α and HIF-1-α were significantly increased in hyperoxia group and H2 O2 groups,and the expression levels of TNF-α and HIF-1-α were higher in hyperoxia group than those in H2O2 groups (P<0. 05). Conclusion The injury of intestinal epithelial cells in hyperoxia environment is accompa-nied by ROS increase. So we make a conclusion that ROS plays an important role in intestinal injury in hyperoxia envi-ronment.