IL-2基因启动子区域 T-384G 基因多态性与中国汉族人肠易激综合征的关系

摘要

Objective To explore the relationships of promoter region T-384G polymorphisms of interleukin-2(IL-2)gene to irritable bowel syndrome(IBS)and serum IL-2 levels.Methods A case-control study was carried out,and 213 IBS patients and 213 age- and sex-matched healthy controls were recruited in this study.IL-2 T-384G polymorphisms and serum IL-2 levels were de-termined by PCR-RFLP and ELISA,respectively.Results The frequencies of TT,TG and GG genotypes were,respectively,77,100 and 36 in IBS group,and 115,86 and 12 in control group. Compared with control group,the frequency of GG genotype significantly increased in IBS group (P <0.05).Compared with TT+TG carriers,serum IL-2 levels significantly increased in GG car-riers(P <0.05).Conclusion GG genotype,a susceptible gene of IBS,is associated with higher levels of serum IL-2.%目的：探讨 IL-2基因启动子区域 T-384G 基因多态性与肠易激综合征（irritable bowel syndrome，IBS）及其血清 IL-2水平的关系。方法采用病例-对照研究方法纳入 IBS 患者（IBS 组，n＝213）及年龄、性别相匹配的健康人群（对照组，n＝213）。以聚合酶链反应-限制性内切酶片段长度（PCR-RFLP）方法分析2组 IL-2基因启动子区域T-384G 基因多态性。以酶联免疫吸附方法（ELISA）测定2组血清 IL-2水平。结果IBS 组及对照组 TT、TG 及GG 基因型分布频数分别为77、100、36及115、86、12。IBS 组 GG 基因分布显著高于对照组（P ＜0．05）。GG 基因型人群血清 IL-2水平显著高于 TT＋TG（P ＜0．05）。结论GG 基因型是 IBS 易患基因，可能与其具有较高的 IL-2表达水平有关。