引起大豆苗期根腐病的大豆拟茎点种腐病菌鉴定

摘要

The 10 fungal isolates causing soybean root rot were identified.The results showed that 10 isolates had similarity to Phomopsis longicolla in morphology.BLAST search results showed that the sequence of P .longicolla strain MP4PL1 1PS (GenBank accession number HQ130441.1)was homologous to the tested strain with a similari-ty of 98% by using universal primers (ITS)for amplification.So,the isolated strain was identified as P .longicolla .%对10个引起大豆根腐病的真菌分离物进行鉴定。结果表明，这些分离物形态学上与大豆拟茎点种腐病菌相似。用 ITS 通用引物进行 PCR 扩增，该菌株与拟茎点种腐病菌 MP4PL11PS (GenBank 登录号 HQ130441.1)同源性达98%。因此，确定该菌株为大豆拟茎点种腐病菌。