果蔗脱毒种苗甘蔗花叶病、黄叶病和宿根矮化病分子检测

摘要

为监测2016-2017年种植的果蔗脱毒种苗脱毒效果,分别采集广州市南沙区和增城区、湛江市麻章区及华南农业大学甘蔗育种基地共83份果蔗脱毒种苗样本,进行甘蔗花叶病毒(SCMV)、高粱花叶病毒(SrMV)和甘蔗黄叶病毒(SCYLV)RT-PCR检测.结果表明SCMV的阳性样本数为3个,阳性检出率3.61%;SrMV的阳性样本数为0;SCYLV的阳性样本数为78个,阳性检出率93.98%.采用常规PCR和巢式PCR技术对采集于广州市增城区和华南农业大学甘蔗育种基地的30份果蔗脱毒种苗样本进行宿根矮化病菌(Lxx)检测,常规PCR检测阳性样本数为0,巢式PCR检测疑似阳性样本数为8,疑似阳性检出率26.67%.本研究采用茎尖组织培养脱毒技术培育的果蔗脱毒种苗能有效脱除果蔗种苗内的SCMV、SrMV和Lxx,但SCYLV的脱除效果有待进一步研究.%To survey the virus elimination effect of virus-free fruit cane seedlings,we collected 83 samples of virus-free seedling from Nansha District and Zengcheng District of Guangzhou,Mazhang District of Zhanjiang and sug-arcane breeding base of South China Agricultural University(SCAU)to detect Sugarsane mosais virus(SCMV), Sorghum mosais virus(SrMV)and Sugarsane yellow leaf virus(SCYLV)by RT-PCR in 2016-2017.The results showed that three positive samples of SCMV was found,with the positive detection rate of 3.61%.The positive sample of SrMV was not found.The number of positive samples of SCYLV was 78,and its positive detection rate was 93.98%.We also collected 30 samples of virus-free seedling from Zengcheng District of Guangzhou and sug-arcane breeding base of SCAU to detect the pathogen(Lxx)caused ratoon stunting disease(RSD)based on nested PCR or conventional PCR.The results showed that conventional PCR failed to detect the positive samples,but nested PCR found 8 suspected positive samples,with the suspected positive detection rate of 26.67%.In this stud-y,the virus-free seedlings developed by techniques of shoot apical meristem culture can effectively remove SCMV, SrMV and Lxx in the fruit cane seedlings,but the removal effect of SCYLV needs further study.