云南大麻品种抗旱性研究初报1

摘要

In this study, the hemp varieties‘Yunma 1’,‘Yunma 5’ and‘Yunwan 6’ cultivated in Yunnan province were used for the drought resistance analysis, with PEG-6000 simulating drought stress and controlled watering in pot culture during the germination and seedling stages respectively.At germination stage, the tolerance concentration of PEG-6000 for germination was from 0 to 15%, but the seed germination was significantly inhibited in the concentration over 20%.And there were significant differences in the drought resistance among different hemp varieties at germination stage.During seedling period, with the increase of stress time and stress level, the growth rate of plant height decreased gradu-ally, the leaf chlorophyll content increased irregularly, and content of small molecular substances, inclu-ding proline and soluble sugar, increased gradually.Superoxide dismutase ( SOD) activity showed a rise-and-fall trend, while malondialdehyde ( MDA) content was a fall-and-rise pattern.These results indicated that all the three hemp varieties were drought resistant in some extent, but‘Yunwan 6’ was the best one, and‘Yunma 5’ was the lowest tolerance one.%本研究选用云南大麻品种云麻1号、云麻5号和云晚6号为材料，分别采用PEG－6000模拟干旱法和盆栽控水法研究大麻萌发期和苗期抗旱性。结果表明：在萌发期，大麻耐受PEG－6000浓度位于0～15％之间，而浓度高于20％则大幅抑制大麻种子萌发。同时，不同大麻品种萌发期的抗旱性存在显著差异。苗期，随着胁迫时间和程度的增加，大麻株高增长速率逐渐降低，叶片叶绿素含量呈不规则增加，脯氨酸和可溶性糖含量逐渐增加，超氧化物歧化酶（ SOD）活性呈先升后降趋势，丙二醛（ MDA）含量则先降低后增加。综合以上萌发期和苗期的指标发现，供试大麻品种均有一定的抗旱能力，其中云晚6号抗旱性最强，云麻5号较弱。