HPLC法测定毛冬青胶囊中具栖冬青苷和毛冬青酸的含量

摘要

Objective: To establish an HPLC method for the determination of pedunculoside and ilexolic acid in Maodongqing capsules. Methods: The separation was carried out at 40 ℃ on an Agilent Zorbax SB-C18 (4.6 mm ×150 mm, 5μm) column with methanol and 0.2% phosphoric acid as a mobile phase in a gradient elution. The flow rate was 1.0 mL·min-1 and the injection volume was 20 μL. The detection wavelength was set at 215 nm. Results: Pedunculoside showed a good linear relationship at the range of 1.22~521.1μg·mL-1 (r=0.9995), the average recovery was 99.24% with RSD 0.68%. Ilexolic acid showed a good linear relationship at the range of 1.23~289.6 μg·mL-1 (r=0.9991), the average recovery was 99.53%with RSD 0.85%. Conclusion: The method is convenient, accurate with good reproducibility and can be used for quality control of Maodongqing capsules.%目的：建立HPLC法同时测定毛冬青胶囊中具栖冬青苷和毛冬青酸含量的方法，并对3个批次毛冬青胶囊中上述两种化合物进行定量分析。方法：Zorbax SB-C18（4.6 mm×150 mm，5μm）色谱柱，流动相为甲醇-0.2%磷酸水溶液，梯度洗脱，检测波长215 nm，柱温40℃，流速1.0 mL·min-1，进样量20μL。结果：具栖冬青苷和毛冬青酸的线性范围分别为1.22~521.1μg· mL-1（r=0.9995）、1.23~289.6μg·mL-1（r=0.9991），加样回收率分别为99.24%（RSD=0.68%）、99.53%（RSD=0.85%）。结论：该方法简便、准确、重复性好，可为毛冬青胶囊提供质量控制依据。