目的：采用Caco-2细胞Transwell模型研究仙茅苷的跨膜转运机制。方法：建立Caco-2细胞Transwell吸收模型，用聚酯碳酸酯膜培养Caco-2细胞21天，形成致密的单层细胞模型。研究浓度、时间、温度、细胞旁路转运及跨膜转运蛋白（P-糖蛋白，多药耐药蛋白，乳腺癌耐药蛋白）在仙茅苷跨膜转运中的作用。结果：仙茅苷在Caco-2细胞模型中的跨膜转运存在一定的浓度及时间依赖性，细胞旁路转运不参与其转运，仙茅苷的外排转运存在一定的能量依赖性，且由P-gp参与。结论：仙茅苷在Caco-2细胞模型上主要以主动转运方式作跨膜转运，且P-gp参与其跨膜转运。%Objec tive: To investigate the intestinal transport mechanism of curculigoside using Caco-2 cell monolayer transwell model. Methods: To develop a Caco-2 cell monolayer transwell model, Caco-2 cells were seeded on the permeable polycarbonate membranes and incubated for 21 days until a compact membrane was developed, and then the effects of concentration, time, temperature, paracellular pathways and efflux transporters (P-glycoprotein, multidrug resistance protein 2, breast cancer resistance protein) on the transport of curculigoside across the Caco-2 cell monolayers were investigated. Results: The results indicated that the intestinal transport of curculigoside was a time- and concentration-dependent active transport, the paracellular pathway was not involved, the efflux of curculigoside was energy dependent, and P-glycoprotein was involved. Conclusion: Curculigoside is transported mainly in an active mode and P-glycoprotein is involved.
展开▼
