1％藜芦碱·血根碱可溶液剂高效液相色谱分析方法研究

摘要

建立高效液相色谱检测1％藜芦碱·血根碱可溶液剂的分析方法.采用乙腈溶解样品,以乙腈和0.05％三氟乙酸水溶液为流动相,使用内填Nova-Pak C18的不锈钢柱和二极管阵列检测器,在230nm波长下对1％藜芦碱·血根碱可溶液剂进行分离和定量分析.藜芦碱在0.20 ～0.79 mg/mL、血根碱在0.17 ～0.83 mg/mL范围内线性关系良好,相关系数均在0.99以上,回收率在99.26％～104.19％之间,标准偏差为0.084.该方法简便、快速,结果准确、可靠,准确度和精密度高,可以实现高效液相色谱对1％藜芦碱·血根碱可溶液剂的分离和检测.%A high performance liquid chromatography method was established for determination of sanguinarine + veratramine 1％ SL.The samples were dissolved with acetonitrile and detected by HPLC with acetonitrile and 0.05％ aqueous trifluoroacetic acid solution as mobile phase,Nova-Pak C18 column and DAD at 230nm.The linear ranges for sanguinarine and veratramine were 0.17 ～ 0.83 mg/mL and 0.20 ～ 0.79 mg/mL,respectively,the correlation coefficients were all above 0.99,the average recoveries for sanguinarine and veratramine ranged from 99.26％ ～104.19％,and the relative standard deviations were 0.084.The method is simple,rapid,accurate and sensitive enough to be suitable for determination of sanguinarine + veratramine 1％ SL.