Kaffir lime leaf extract mediated synthesis, anticancer activities and antibacterial kinetics of Ag and Ag/AgCl nanoparticles

摘要

A facile phytosynthesis of Ag/AgCl and Ag nanoparticles (NPs) was developed using a crude water extract of Citrus hystrix DC (kaffir lime) leaves.The phytochemical and chloride contents of the extract,and various synthesis parameters were studied.The obtained Ag/AgCl and Ag NPs were of the cubic phase and mostly spherical in shape.Particle sizes of the yielded NPs were distributed in a narrow range with an average size of 20 and 38 nm for the Ag/AgCl and Ag NPs,respectively.The reducing function of the phytochemicals and the stabilizing ability of gelatin were demonstrated.The NPs showed significant antiproliferative activities against the carcinoma HCT 116 and adenocarcinoma Caco-2 colorectal cell lines,but had a negative effect toward human fibroblasts.The NPs exhibited excellent antibacterial activities against Escherichia coii,Staphylococcus aureus,Pseudomonas aeruginosa and Bacillus cereus.The inactivation kinetics obtained in the presence of the NPs showed a biphasic behavior that can be explained by the Cerf model.