miR-595在生长期下颌前突患者血清中的表达水平

摘要

Objective:To study the expression level of miR⁃595 in serum of patients with mandibular prognathism during growth pe⁃riod and its relationship with mandibular growth and ossification. Methods:The research chose 83 skeletal classⅢ malocclusion ( 23 mixed dentition, 60 early permanent dentition) and 92 normal controls ( 24 mixed dentition, 68 early permanent dentition) , using RT⁃PCR to detect miR⁃595 expression levels in two groups and TargetScan software to predict its possible target genes. Results:Expression of miR⁃595 in mandibular prognathism group was down regulated. There was negative correlation between miR⁃595 and mandibular growth with statistical differences ( P<0.01) . Conclusions:miR⁃595 might have inhibitory effect on mandibular growth and ossification through target gene.%目的：探讨microRNA⁃595（ miR⁃595）在生长期骨性下颌前突患者血清中的表达水平及其与下颌骨生长的关系。方法：选取骨性下颌前突患者83例（替牙列23例，早期恒牙列60例）及正常对照92例（替牙列24例，早期恒牙列68例），用实时荧光定量PCR法检测两组样本血清中miR⁃595的表达情况，通过Target Scan软件预测它可能的靶基因，并对测量结果进行统计学分析。结果：与对照组相比，下颌前突组miR⁃595的表达量下调，与下颌骨的生长量呈负相关，有明显统计学差异（ P＜0．01）。结论：miR⁃595可能通过靶基因对下颌骨生长起负调控（抑制）作用。