目的:探讨高血糖大鼠牙髓干细胞(DPSCs)增殖及成牙/成骨分化能力.方法:选取3个月龄糖尿病模型动物GK大鼠及正常对照Wistar大鼠,测定尾静脉血糖值,待血糖稳定1周后,通过酶消化法分离培养两组大鼠DPSCs,通过MTT法测定两组大鼠DPSCs生长曲线,实时定量RT-PCR及Western blot检测两组大鼠DPSCs牙本质基质蛋白1(DMP1)、Osterix(Osx)、骨钙素(OCN)等成牙/成骨向分化指标的表达.结果:GK大鼠餐后2小时尾静脉血糖值稳定在20.1~22.3 mmol/L,Wistar大鼠为5.8~6.3 mmol/L.MTT结果显示,GK大鼠DPSCs增殖能力从培养第3天到第7天均较Wistar大鼠DPSCs低(P<0.05).实时定量RT-PCR及Western blot检测结果显示GK大鼠DPSCs成牙/成骨向分化指标的表达明显较正常对照Wistar大鼠低.结论:高血糖大鼠DPSCs增殖能力及成牙/成骨向分化能力均较正常大鼠低.%Objective:To explore the proliferation and odonto/osteogenic potential of DPSCs separated from GK rat with hyperglyce-mia. Methods:The 3-month-old GK rat(one diabetic model rat)and Wistar rat were selected. The DPSCs were isolated and cultured when the blood glucose level had been stable for 1 weeks. MTT assay was applied to estimate the proliferation of the two DPSCs. The odonto/osteogenic markers(OCN, OSX and DMP1)of the two DPSCs were measured by real-time RT-PCR and Western blot. Re-sults:The level of blood glucose in GK rats remained about 20.1~22.3 mmol/L(high glucose)and the level of blood glucose of Wist-ar rats was 5.8 ~ 6.3 mmol/L(normal glucose level). MTT assay showed the proliferative activity of DPSCs from GK was significantly lower than that of the Wistar rat. Moreover,the expressions of OCN, OSX and DMP1 in DPSCs of GK rats were significantly weaker than those in Wistar rats. Conclusions: The DPSCs from the donor with hyperglycemia or diabetes exhibited the weaker proliferation and odonto/osteogenic potential.
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