通过设置不同温度、盐度、pH值和摄食状态的方法,研究凡纳滨对虾在不同条件下的无机磷、亚硝酸氮、硝酸氮、氨氮的释放率.结果表明,温度、体重、盐度、pH值和摄食状态对其代谢存在影响.无机磷、亚硝酸氮、硝酸氮、氨氮的释放率与体重呈负相关;在20-30℃的温度范围内,随着温度的上升,凡纳滨对虾磷、亚硝酸氮、氨氮的释放率均上升,硝酸氮的释放率在25℃时最高;在10-31的盐度范围内,随着盐度的升高,凡纳滨对虾[(5.341±0.041)g]磷的释放率升高,亚硝酸氮、硝酸氮、氨氮的释放率均降低;在7.5-9.0的pH值范围内,随着pH值的升高,凡纳滨对虾[(5.355±0.021)鲥磷的释放率明显下降,亚硝酸氮的释放率上升,氨氮的释放率在pH值为8.5时达到最大值,硝酸氮的释放率在pH值为8.5时达到最小值;凡纳滨对虾[(0.344±0.063)g]摄食配合饲料,饱食状态下磷、亚硝酸氮、硝酸氮、氨氮的释放率比饥饿状态下分别提高了38.5%、131.3%、93.4%、93.3%.表明摄食时蛋白质代谢增加显著.%We measured the release rates of inorganic phosphorus, nitrite nitrogen, nitrate nitrogen and ammonia of Litopenaeus vannamei under different conditions. Temperature, weight, salinity, pH and feeding status affected metabolism.The release rates of phosphorus, nitrite nitrogen, nitrate nitrogen and ammonia nitrogen were negatively correlated with the body weight. In the range of 20—30℃, as the temperature rose, the release rates of phosphorus, nitrite nitrogen and ammonia were increased; the release rate of nitrate nitrogen was highest at 25 ℃. In the range of 10-31, as the salinity increased, the release rate of phosphorus of L. vannamei [(5.341±0.041)g] was increased, the release rates of nitrite nitrogen,nitrate nitrogen, and ammonia nitrogen were decreased. In the range of 7.5-9.0, as the pH increased, the release rate of phosphorus by L. vannamei [(5.355±0.021)g] was decreased significantly; and the release rate of nitrite nitrogen was increased. When pH was 8.5, the release rate of ammonia nitrogen reached the maximum, the release rate of nitrate nitrogen reached the minimum. Compared with the state of hunger, in the condition of feeding formula foodstuff, the release rate of phosphate, nitrite nitrogen, nitrate nitrogen, and ammonia nitrogen by L. vannamei [(0.344±0.063)g] were increased by 38.5％, 131.3％, 93.4％, and 93.3％, respectively. Results show that the protein metabolism increases significantly in feeding.
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