目的：在实验室酶法提取的基础上，优化苦豆草生物碱酶法提取小试工艺条件。方法以苦豆草生物碱的提取率为指标，在小试条件下优化提取次数、料液比、提取时间，并与传统提取法比较。结果酶法提取最佳的小试工艺条件为：酶解时间4h，溶剂pH＝6，温度为50℃，加酶量为1∶1000，酶解后，加入盐酸至浓度为4mL·L -1，料液比1∶16，提取2次，第1次2h，第2次1 h。苦豆草生物碱的提取率达到2．17％结论实验室提取工艺与小试生产存在差异，经条件优化基本达到实验室提取生物碱的提取率。%Objective Based on the laboratory enzymatic extraction method ,to optimize small-scale process conditions of alkaloids enzymatic extraction from Sophora alopecuroides L ..Method By using the alkaloids extraction rate as index ,extraction time , solid-liquid ratio and extraction times were optimized in small-scale process ,and the results were compared with the traditional ex-traction method .Results The optimal small-scale process conditions of enzymatic extraction were as follows :solvent pH 6 ,a-mounts of enzyme (1∶1 000) ,enzymatic hydrolysis at temperature 50 ℃ for 4 h .Then hydrochloric acid was added to the con-centration of 4 mL · L -1 ,solid-liquid ratio 1∶16 ,extracting for 2 times .The first time extracted 2 h ,and the second time ex-tracted 1 h .The extraction rate of alkaloids from Sophora alopecuroides L .was 2 .17% .Conclusion Although the small-scale technology was different from the laboratory enzymatic extraction ,the extraction rate was almost same .
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