氨基丁酸联合卡泊芬净抗白色假丝酵母菌生物被膜协同作用研究

摘要

目的：探讨氨基丁酸联合卡泊芬净抗白色假丝酵母菌生物被膜的协同作用。方法利用白色假丝酵母菌标准菌株SC5314，采用生物被膜形成实验，分为空白对照组、氨基丁酸单用组、卡泊芬净单用组、氨基丁酸联合卡泊芬净组，对比各组生物被膜形成情况。采用XT T还原法测定氨基丁酸、卡泊芬净单用以及氨基丁酸联合卡泊芬净对成熟生物被膜细胞代谢活性的抑制作用。采用YNB培养基菌丝形成实验，考察氨基丁酸与卡泊芬净合用是否具有协同抑制菌丝形成的作用。结果卡泊芬净0．1μg · mL －1联合氨基丁酸0．1μmol · L －1对白色假丝酵母菌SC5314生物被膜的形成具有显著的抑制作用。此外，XT T还原法测定氨基丁酸6．25μmol · L －1联合卡泊芬净0．1μg · mL －1时降低被膜细胞代谢活性的效率能够达到约15％。采用YNB培养基形成菌丝，氨基丁酸6．25μmol · L －1联合卡泊芬净0．1μg · mL －1对白色假丝酵母菌SC5314菌丝形成能力有显著的抑制作用。结论氨基丁酸联合卡泊芬净表现出显著的体外协同抗白色假丝酵母菌标准菌株SC5314生物被膜作用。%Objective To investigate the enhanced effect of aminobutyric acid (GABA) combined with caspofungin on biofilm forma‐tion of Candida albicans .Methods Standard stains of Candida albicans SC5314 were used in this study .The group of caspofun‐gin with GABA comparing with the group of GABA or caspofungin alone were evaluated by observing the prevention of biofilm formation .Candida albicans SC5314 were cultured in YNB medium to induce the formation of hyphae and the metabolic activity was determined by XTT reduction assay .Results The group of caspofungin (0 .1μg · mL -1 ) showed no inhibition against biofilm formation of Candida albicans ,but caspofungin (0 .1 μg · mL -1 ) with GABA (0 .1 μmol · L -1 ) showed apparent inhibition a‐gainst biofilm formation of Candida albicans .The metabolic activity of caspofungin 0 .1 μg · mL -1 with GABA 6 .25 μmol · L -1 reduced to about 15% .Conclusion The combination of caspogungin with GABA possessed an enhanced effect against the biofilm formation of Candiada albicans SC5314 in vitro .