广枣提取物大鼠肠外翻的吸收研究

摘要

目的 研究广枣提取物中2种主要代表性成分没食子酸和原儿茶酸在大鼠不同肠段、不同时间的吸收规律.方法 构建大鼠肠外翻模型,应用HPLC法测定广枣提取物肠吸收液中没食子酸和原儿茶酸含量,计算其吸收参数,并分析其在大鼠小肠不同部位、不同时间的吸收特征.结果 2.5 h为肠外翻最终检测时间点,在建立的分析色谱条件下,没食子酸和原儿茶酸色谱峰处台氏液和广枣提取物中其他成分对其无干扰,专属性良好.广枣提取物中原儿茶酸和没食子酸在各肠段均为线性吸收,r2值均达到0.90以上,不同肠道部位的累计吸收结果为:十二指肠>空肠>回肠>结肠.结论 广枣提取物中原儿茶酸和没食子酸在肠道的吸收特征符合零级吸收速率,其最佳吸收部位为十二指肠.%Objective To research the intestinal absorption characteristics of representive ingredients including gallic acid (GC) and protocatechuic acid (PA) in Choerospondiatis Fructus extracts at different intestinal sections and different time.Methods The everted gut sac model was established,and the contents of GC and PA in the intestinal samples were determined by high performance liquid chromatography (HPLC),with calculating the absorption parameters and analysis of the absorption characteristics in the different parts of rat small intestine at different time.Results The final determination point for everted gut sac was 2.5 h,Tyrode buffer solution and other ingredients had no interference to the chromatographic peak of GC and PA.Both GC and PA were linear absorption in various intestinal segments,and the square of the correlation coefficient (r2) value was more than 0.90.The advantageous accumulative absorption of different intestinal parts were duodenum,jejunum,ileum and colon.Conclusion Both GC and PA in the intestinal absorption characteristics were accorded with zero order absorption rate,and duodenum was the most absorpted part.