Objective To develop a determination method for isochlorogenic acid B,isochlorogenic acid A and isochlorogenic acid C in Artemisia scoparia extrects by HPLC.Methods The HPLC separation was performed on a Shim-pack VP-ODS column (250 mm×4.6 mm,5 μm) in a gradient elute mode with a mixture consisting of acetonitrile and 0.036 mol·L-1 phosphate acid solution at 30 ℃.The flow rate was 1.0 mL·min-1 and the detection wavelength was set at 327 nm.Results The calibration curves were linear in the ranges of 0.010 3-0.309 mg·mL-1,0.009 7-0.291 mg·mL-1 and 0.010 5-0.315 mg·mL-1 for isochlorogenic acid B,isochlorogenic acid A and isochlorogenic acid C.The average recoveries (n=6) were 99.23%,101.30% and 100.63%,RSD were 1.59%,0.83% and 1.23%,respectively.The properties of sample solution kept steady within 32 h.Conclusion The method is simple,accurate and reproducible,which can be used for the quality control of Artemisia scoparia extrects.%目的 建立滨蒿提取物中3,4-二咖啡酰奎宁酸、3,5-二咖啡酰奎宁酸和4,5-二咖啡酰奎宁酸的含量测定方法.方法 采用HPLC法测定,色谱条件:Shim-pack VP-ODS色谱柱(250 mm×4.6 mm,5 μm);流动相:乙腈-0.036 mol·L-1磷酸二元梯度洗脱;流速:1.0 mL·min-1;柱温:30 ℃,检测波长:327 nm.结果 3,4-二咖啡酰奎宁酸、3,5-二咖啡酰奎宁酸和4,5-二咖啡酰奎宁酸分别在0.010 3~0.309,0.009 7~0.291和0.010 5~0.315 mg·mL-1范围内与峰面积呈良好的线性关系(r=0.999 9);加样回收率分别为99.23%,101.30%和100.63%;RSD值分别为1.59%,0.83%和1.23% (n=6);样品溶液在32 h内稳定.结论 该方法操作简便,重复性好,可用于滨蒿提取物的质量控制.
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