活性绿19修饰花生壳粉微球的制备及对溶菌酶的吸附

摘要

A new bio-adsorbent from natural product peanut shell was prepared with epichlorohydrin and Reactive Green 19 (RG19) as crossliking and modification agents,respectively.The adsorption behavior of the modified product for lysozyme in the different conditions, including pH, initial concentration of lysozyme, contact time, temperature, and NaC1 concentration,was investigated by comparison with unmodified one.The lysozyme adsorption capacity of RG19 modified peanut shell was 149.6 ag·g-1 ,when 10 mL lysozyme solution was absorbed with 25.0 mg modified peanut shell at pH 7.4, and stirring for 4 h.However, the adsorption capacity of unmodified peanut shell just was 23.6 mg · g-1, and the former is 6.3 times of the latter.The seperation and purification of lysozyme from egg white were achieved.The lysozyme was purified 31.0-fold in a single step with a recovery 64.2％.In addition,the repeat usefulness of this new-style absorbent is better.%以天然产物花生壳粉作为基质,环氧氯丙烷为交联剂,活性绿19(Reactive Gteen 19,简称RG19)为修饰剂,制备了新型的生物吸附剂RG19修饰花生壳粉微球,比较了RG19修饰花生壳粉前后对溶菌酶的吸附性能,包括吸附溶液的pH,溶菌酶的初始浓度,吸附时间,温度及NaCl的浓度对吸附的影响.结果表明,用25.0 mgRG19修饰花生壳粉微球处理溶菌酶溶液10 mL,pH值7.4,吸附时间4 h的条件下,对溶菌酶的吸附量是149.6mg·g-1,其酶活力保持率为96.4%,而未修饰的花生壳粉微球对溶菌酶的吸附量只有23.6 mg·g-1,修饰后是修饰前的6.3倍.在上述条件下从鸡蛋清中分离纯化溶菌酶,纯化倍数为31.0,收得率为64.2%.而且该吸附剂的复用性好.