Silica-gel column,macroporous resin column, Sephadex LH-20 column and reversed phase C18 column were used to separate and purify chemical constituents of Pedicularis longiflora Rudolph, var. tubiformis (Klotz). Tsoong. Seven compounds were obtained. They were identified as luteolin (1) .apigenin (2) ,chrysoeriol(3) ,3,5,7-trihydroxy-3', 5'-ditnethoxyl flavone (4) ,verbascoside (5) ,isoverbascoside (6) and luteolin-4'- O-β-D-glucoside (7) using H NMR and I3C NMR. These 7 compounds were separated from Pedicularis longiflora Rudolph, var. tubiformis ( Klotz). Tsoong for the first time. The results of this study can be used for further researches of this traditional Tibetan medicine.%采用硅胶柱、大孔树脂柱、Sephadex LH-20柱、反相C18柱等柱层析手段,对斑唇马先蒿的化学成分进行了系统的分离纯化,得到了7个化合物,其1H NMR和13C NMR鉴定结果为木犀草素(luteolin,1)、芹菜素(apigenin,2)、金圣草黄素(chrysoeriol,3)、3,5,7-三羟基-3’,5’-二甲氧基黄酮(3,5,7-trihydroxy-3’,5’-dimethoxyl flavone,4)、毛蕊花苷(verbascoside,5)、异毛蕊花苷(isoverbascoside,6)、木犀草素4’-O-β-D-葡萄糖苷(luteolin-4’-O-β-D-glucoside,7).这7个化合物均首次从该植物中分离得到,为藏药斑唇马先蓠的深入研究提供了基础.
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