冬瓜粗提物对小鼠紫外线晒伤皮肤的修复作用

摘要

To evaluate the rehabilitation effects of Benincasa hispida extract on sunburn mice skin by urtraviolet radiation.The mice model was developed by UV radiation,and the model mice were divided into normal control,negative control,positive control (lidocaine) and experimental group (freeze drying group) randomly.The mice in positive control and experimental group were applied with lidocaine and B.hispida extract twice a day respectively,both normal group and negative group were applied with saline twice a day.Then,executed the mice on 0,1,3,7,14 d respectively.The back skin was separated to make biopsy and western-blot assay.The expressions of growth factor of VEGF and FGF-2 were higher in positive control and experimental group than those in other groups,and significant difference was detected between negative control group and experimental group.The pathological analysis results showed that the number of blood vessels,fiber cells and fibroblasts in experimental group was significantly higher than those in negative group.B.hispida extract enhanced the expressions of VEGF and FGF-2,and promoted wound healing.%为探讨冬瓜(Benincasa hispida)粗提物对小鼠紫外线晒伤皮肤的修复作用,建立小鼠紫外线晒伤模型.观察冬瓜粗提物对晒伤小鼠内源性血管内皮生长因子(VEGF)及碱性成纤维细胞生长因子(FGF-2)表达的影响.冬瓜提取物组(浓度均为31 mg/mL),药物组(利多卡因氯已定气雾剂),正常对照组和阴性组(生理盐水),每天2次涂药并观察创面,分别在0、1、3、7、14d处死小鼠,取皮肤组织,做病理切片,取晒伤组织提取蛋白并检测VEGF、FGF-2的表达状况.3～7d时,阳性对照组和实验组的VEGF和FGF-2的表达明显高于阴性对照组和正常组,且阳性组与实验组VEGF和FGF-2表达水平有显著差异.病理切片显示实验组炎性细胞和血管数量明显多于阴性对照组,且两组间差异明显.即冬瓜粗提物能提高皮肤组织的生长因子VEGF和FGF-2的表达,促进晒伤皮肤的愈合.