西洋参中8种人参皂苷类成分的UPLC-MS/MS定量分析

摘要

建立超高效液相色谱串联三重四级杆质谱法(UPLC-MS/MS)同时测定西洋参中8种人参皂苷类成分(人参皂苷Rb1、人参皂苷Rb2、人参皂苷Rb3、人参皂苷Re、人参皂苷Rc、人参皂苷Rd、人参皂苷Rg1和拟人参皂苷F11)的定量分析方法.采用Waters Acquity BEH C18柱(100 mm ×2.1 mm,1.7 μm)色谱柱,流动相为0.1％甲酸水(A)-乙腈(B),梯度洗脱,流速0.25 mL/min,柱温35℃.电喷雾电离源(ESI),采用多反应检测模式(MRM),以保留时间及定性离子对之间的相对丰度定性,以定量离子对峰面积进行定量.定量分析西洋参中8种人参皂苷类成分在考察的浓度范围内呈良好的线性关系(r＞0.99);回收率和RSD分别在95.65％～103.34％,0.38％～4.33％.本研究所建立的同时测定西洋参中8种皂苷类成分的UPLC-MS/MS定量分析方法简便、快捷、准确,可为综合评价西洋参的质量提供参考.%To establish an ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous determination of 8 major compounds (Ginsenoside Rb3,GinsenosideRc,Ginsenoside Rb1,Ginsenoside Rb2,Ginsenoside Rd,Ginsenoside Re,Ginsenoside Rg2,Pseudoginsenoside F11) in Panax quinquefolium.Using Waters BEH C18 column (2.1 mm × 100 mm,1.7 μm) with a gradient solvent system of acetonitrile-0.1％ formic acid in water as mobile phases,flow rate:0.25 mL/min,column temperature:35 ℃.Multiple reactions monitoring (MRM) mode was used as detection mode with electro-spray ionization (ESI) source.The retention time and detected relative abundance of ion pairs were used for qualitative determination,the detected peak areas of ion pairs were used for quantitative determination.Under the optimized chromatographic conditions,good separation for 8 targeted compounds were obtained.Satisfactory linearity was achieved with wide linear range and fine determination coefficient (r ＞ 0.999),the over-all recoveries were ranged from 95.65％-103.34％ with the RSD ranging from 0.38％ ～4.33％.It is the report about simultaneous analysis of 8 major ginsenosides components in P.quinquefolium by using UPLC-MS/MS method,which affords highly sensitive,specific,speedy and efficient method for quality control of P.quinquefolium.