中医痰证与载脂蛋白A5基因-1131T》C多态性的关系研究

摘要

Objective It is to explore the relationship between the polymorphisms of apolipoprotein A5(APOA5) gene( -1131T >C) and phlegm stagnation syndrome ( PSS). Methods Patients of PSS ( n = 131) and healthy controls ( m 74 ) were grouped and compared in this study. Using polymerase chain reaction restriction fragment length polymorphisms (PCR-RFLP) technique to analyze the APOA5( -1131T>C) genotypes. Serum lipids, blood pressure(BP) and body mass index(BMI) in the patients and controls were also measured . Results The frequencies of TT , TC, CC genotypes for the APOA5 - 1131T > C polymorphism were 37.07% (n = 76) , 45. 85% (n=94) and 17.07% (n=35). The frequency of T allele was 60.00% and the frequency of C allele was 40.00%. The serum triglyceride ( TG) level of - 1131 CC genotype carries were significantly higher than - 1131TT genotype carries (P <0. 01). Patients-controls research and multinomial -logistic analysis indicated that -1131C was a risk factor of the PSS. Conclusion The -1131C allele of APOA5 gene is possibly the independent risk factor of the SPS. The - 1131CC genotype is possibly associated with the higher level of serum triglyceride .%目的 探讨载脂蛋白A5(ApoA5)-1131T>C基因多态性与中医痰证的关系.方法 运用聚合酶链反应-限制性片段长度多态性分析技术对湖南地区131例痰证患者及74例健康人的ApoA5-1131T>C基因型进行检测,同时检测所有研究对象的血脂、血压及体质量指数.结果 205例受检者中,ApoA5-1131T>C位点TT型76例(37.07%),TC型94例(45.85%),CC型35例(17.07%),T等位基因频率60.00%,C等位基因频率40.00%.CC型血清三酰甘油水平明显高于TT型(P<0.01).病例-对照研究及多因素Logistic 回归分析均支持ApoA5-1131C是痰证发生的独立危险因素.结论 ApoA5-1131C可能是痰证发生的独立危险因素.ApoA5-1131CC型可能与血清三酰甘油水平升高有关.