温阳振衰颗粒对阿霉素致心肌细胞损伤MEF2表达的影响

摘要

目的 观察温阳振衰颗粒含药血清对H9 C2心肌细胞损伤模型肌细胞增强因子2(MEF2)表达的影响.方法 实验分为正常对照组、H9C2损伤组、5％含药血清组和10％含药血清组.H9C2损伤组的细胞培养基中加入2.67μmol/L的阿霉素(ADR),两含药血清组培养基中加入2.67μmol/L的ADR和5％,10％温阳振衰颗粒含药血清,共培养44 h.检测实验末各组H9C2心肌细胞中p-MEF2、MEF2表达量.结果 各损伤组细胞存活率和p-MEF2表达量均明显低于正常对照组(P均<0.05),5％含药血清组和10％含药血清组细胞存活率和p-MEF2表达量均明显高于H9C2损伤组(P均<0.05),且10％含药血清组均明显高于5％含药血清组(P均<0.05);各组间H9C2心肌细胞MEF2表达量比较差异均无统计学意义(P均>0.05).结论 温阳振衰颗粒能抑制MEF2蛋白的过度去磷酸化,这可能是其治疗慢性心力衰竭的重要机制之一.%Objective It is to observe the effect of Wenyang Zhenshuai Granule on expression of myocyte enhancer fac-tor 2 (MEF2) in adriamycin induced H9C2 myocardial cell damage model. Methods H9C2 myocardial cells were divided into normal control group, H9C2 damage group, 5％ drug serum group and 10％ drug serum group. In the H9C2 damage group, 2. 67 mol/L of adriamycin (ADR) was added to the cell culture medium; in the two drug serum group was added with 2. 67 mol/L ADR and 5％, 10％ drug serum of Wenyang Zhenshuai Granule, and co cultured for 44 hours. The ex-pression levels of p-MEF2 and MEF2 in H9C2 myocardial cells were detected at the end of the experiment. Results The cell survival rate and p-MEF2 expression level in each damage group were significantly lower than those in the normal con-trol group (all P<0. 05), the cell survival rate and p-MEF2 expression level in the 5％ drug serum group and the 10％drug serum group were significantly higher than those in the H9C2 damage group (all P<0. 05), and those of the 10％drug serum group were significantly higher than the 5％ drug serum group (all P<0. 05). There was no significant differ-ence in the expression of MEF2 between H9C2 cells in all groups (P>0. 05). Conclusion Wenyang Zhenshuai Granule can inhibit the excessive dephosphorylation of MEF2 protein, this may be one of the important mechanisms in the treatment of chronic heart failure.