香味剂中大茴香脑、d-柠檬烯、香芹酚含量测定的研究

摘要

An effective method was developed to determine anethole、d-limonene and carvacrol in fodder flavouring agent by GC. The anethole、d-limonene and carvacrol were extracted from fodder flavouring agent with acetone and were determined by GC. Recoveries of anethole、d-limonene and car-vacrol were 96.2%~101.5%, 97.1%~102.5% and 96.5%~99.8%.Relative standard deviations were 1.3%, 1.5% and 1.1%. The lower determination limits were 50mg/kg. The method is effective and accurate for determining anethole, d-limonene and carvacrol in fodder flavouring agent.%本研究建立了气相色谱法测定饲料香味剂中大茴香脑、d-柠檬烯、香芹酚的方法。试样经丙酮提取后，用GC测定大茴香脑、d-柠檬烯和香芹酚。大茴香脑、d-柠檬烯和香芹酚的线性范围为1.0～500.0μg/mL。大茴香脑、d-柠檬烯和香芹酚在浓度为1～10 g/kg添加水平时的回收率分别在96.2%～101.5%、97.1%～102.5%和96.5%～99.8%，RSD分别为1.3%、1.5%和1.1%。方法定量限为50 mg/kg。本方法对饲料香味剂中大茴香脑、d-柠檬烯和香芹酚的检测分析准确性高、重现性好。