秦岭凹叶厚朴内生真菌研究初报

摘要

Objective:Through the study on the endophytic fungus of the Magnolia officinalis subsp.biloba,in order to enrich the diversity of endophytic fungus of the Magnolia officinalis subsp.biloba,to promote the development of research on the medicinal plant resources.Methods:Isolated the endophytic fungus from Qinling M.officinalis subsp.biloba with Microbiological methods,Take the Microscopic morphology observation to identificat.Results:34 strains of endophytic fungi were isolated from Qiuling M.officinalis subsp.biloba,among them 14 strains were isolated from Dujiangyan of Sichuan,The Cladosporium is dominant bacterial community.20 strains were isolated from Hanzhong of Shaanxi,the Fusarium is dominant bacterial community.The appraisal result is:1 class 3 orders 4 families 9 genus.aspergillus is 3 strains,penicillium is 1strain,Botrytis is 2 strains,Gliocladium is 1strain,Fusarium is 16 strains,Cladosporium is 5 strains,Alternaria is 1 strain,Rhizoctonia is 2 strains,Phacodium is 3 strains.Conclusion:The endophytic fungi of M.officinalis subsp.biloba have rich population diversity,Based on available for using the Component of M.officinalis subsp.biloba and protecting the resources of M.officinalis subsp.biloba.%目的:通过对凹叶厚朴内生真菌的鉴定研究,进一步丰富凹叶厚朴内生真菌种群的多样性,为更加合理有效的利用药用植物资源提供依据.方法:本文通过微生物方法从秦岭凹叶厚朴中分离纯化内生真菌,采用显微形态观察法对分离得到的内生真菌进行初步鉴定.结果:对秦岭凹叶厚朴内生真菌进行分离纯化及初步鉴定,共分离得到内生真菌34株,其中四川都江堰共分离14株,优势菌群为枝孢属;陕西汉中共分离20株,优势菌群为镰刀菌属.初步鉴定结果为:1纲,3目,4科,9属.其中曲霉属3株,青霉属1株,葡萄孢属2株,胶帚霉属1株,镰刀菌属16株,枝孢属5株,交链孢属1株,束丝核菌属2株,组丝核菌属3株.结论:凹叶厚朴内生真菌的种群存在着极其丰富的多样性特征,为合理利用凹叶厚朴药用成分及保护凹叶厚朴药用植物资源提供理论依据.