荷兰铁组织培养研究

摘要

对荷兰铁组织培养方法进行研究，结果表明：荷兰铁顶芽经消毒处理后接种到MS＋6-BA2．0mg／L＋NAA0．1mg／L培养基中，可诱导出愈伤组织和不定芽：在1／2MS＋6-BA1．0mg／L＋NAA0．1mg／L培养基中继代培养，扩增倍数（MR值）约2．2倍；将继代培养芽丛中高3cm以上的不定芽接种到生根培养基1／2MS＋IAA0．3mg／L中，30d左右生根率达98．2％；炼苗20-30d后移栽，成活率迭95％以上。%Abstract The tissue culture of Yucca elephantipes was studied. The results showed that the terminal bud of Yucca elephantipes was planted in the culture medium MS+6-BA 2.0 mg/L+NNA 0.1 mg/L after being disinfected ,then the callus and adventitious bud were induced. The expansion rate was 2.2 after being subcultured in 1/2MS+6-BA 1.0 mg/L+NAA 0.1 mg/L. Then the subculture buds long than 3 cm were inoculated in rooting medium 1/2MS+IAA 0.3 mg/L, and the rooting rate reached 98.2% after 30 days. The survival rate of transplant was high than 95% after 20-30 clays adaptation.