一株苹果内生枯草芽孢杆菌BS10的发酵条件优化研究

摘要

以苹果内生枯草芽孢杆菌BS10为试验菌株进行发酵培养基的优化,通过单因素试验选出最佳的碳源和氮源,通过正交试验筛选出最佳C/N比,对pH值、发酵温度和接种量进行了研究.结果表明,碳源选用麦芽糊精,氮源选择豆饼粉,初始pH值7.5,温度33℃、接种量为4%为最佳发酵条件,发酵活菌数为1.03×1010 cfu/mL,比原始配方提高了1.94倍.%Apple endobacteria Bacillus subtilis BS10 was studied to optimize fermentation medium.The best carbon source and nitrogen source were selected through single factor experiment,while the best C/N ratio was selected through orthogonal test.And the pH value,fermentation temperature and inoculation quantity were also studied.The results showed that maltodextrin was the best carbon source and soybean cake powder was the best nitrogen source. pH value 7.5,fermentation temperature 33℃,inoculation quantity 4%was the best fermentation condition. Bacillus subtilis BS10 could reach 1.03×1010 cfu/mL,which was 1.94 times higher than the control.