抗原修复在陈旧石蜡切片乳腺珠蛋白免疫组化检测中的应用

摘要

Objective To develop an antigen retrieval method for detection of human mammaglobin ( hMAM) immuno-histochemcal staining in old paraffin-embedded specimens .Methods The tissue sections in test group were put into dis-tilled water after deparaffinization and then moved into citric acid buffer ( pH 3.5) for 10-15 min.The other two meth-ods,microwave method and high pressure cooker method ,were compared as control groups at the same time .Finally, immu-nohistochemistry SP method was used to check the antibody in the sections .Results The color appearance in the test group (pH 3.5 citric solution) was better than that of microwave oven and high pressure cooker groups .In the test group, tissue sections were not easily cast off from the slices .Conclusion In this study,we have established a new and simple antigen retrieval method which will contribute to immunohistochemistry technology .%目的：探索一种简便有效的抗原修复方法，用于陈旧切片的人乳腺珠蛋白（human mammaglobin，hMAM）免疫组化检测。方法将乳腺癌患者陈旧乳腺组织石蜡切片脱蜡至水，按常规免疫组化步骤进行检测。其中，实验组直接将切片浸入pH 3．5的柠檬酸盐缓冲液中室温修复10～15 min；同时以微波和高压加热修复法处理相应患者石蜡切片作为对照，最后以SP检测系统检测抗原修复及与抗体反应的效果。结果实验组与高压组和微波组比较，修复效果较好，且无组织脱片与折损现象。结论建立了一种利用陈旧组织切片检测乳腺珠蛋白的简便、有效的抗原修复方法，并能很好地防止脱片现象发生。