快速上浮脱险和潜水减压病大鼠肺组织miR-16及miR-146 a表达变化

摘要

Objective To study the expression levels of microRNA (miR)-16 and miR-146a in rat lungs of decompres-sion sickness (DCS) caused by fast buoyancy ascent escape or diving .Methods At 0.5 h after fast buoyancy ascent es-cape or diving, the pathological changes in rat lungs and expression levels of miR-16,and miR-146a were detected by re-verse transcription-quantitive polymerase chain reaction and compared with normal control group .Results The pathological characteristics of lungs in two DCS groups were tissue damage .At 0.5 h after DCS caused by fast buoyancy ascent escape , the lung tissue expression levels of miR-16 and miR-146a did not significantly change compared with normal control and diving DCS groups ,but the rat lung tissue expression level of miR-146 a in diving DCS group was obviously increased , com-pared with normal control group .Conclusion miR-146a may play a role in post-transcriptional regulation in the process of diving DCS .%目的：对快速上浮脱险减压病（DCS）和潜水DCS大鼠肺组织microRNA（miR）-16、miR-146a表达变化进行对比研究。方法快速上浮脱险致DCS后0．5 h对大鼠肺组织进行病理学检查和实时定量PCR检测miR-16、miR-146 a表达水平，并与潜水DCS组、正常对照组对比。结果与正常对照组比较，潜水DCS组和快速上浮脱险致DCS组大鼠肺组织出现明显肺损伤特征；潜水DCS组大鼠肺组织miR-146a表达水平显著升高；快速上浮脱险致DCS组大鼠肺组织miR-16、miR-146a表达水平未见显著改变。结论 miR-146a可能在潜水减压病大鼠肺组织损伤过程中发挥了一定的转录后调控作用，具体机制还有待进一步研究。