Objective To investigate the changes in serum levels of nitric oxide ( NO) and endothelin ( ET) in type 2 diabetes patients with vascular complications, and to analyze the relationship between these levels and risk factors.Method We selected 98 cases of type 2 diabetes patients.Based on the grouping criteria, the patients were divided into diabetic patients with vascular complications ( group A,49 cases) and those without ( group B,49 cases) .In addition, 44 age-and body mass index-matched healthy cases were selected for control(group C).Height, weight, blood pressure, duration of diabetes, fasting blood glucose, hemoglobin A1c ( HbA1c), blood lipids, and serum levels of NO and ET-1 of all the patients were recorded.Results The NO levels of the two groups with diabetes mellitus were significantly lower than in group C[(43.87 ±12.05)and (53.29 ±11.75)μmol/L versus (66.08 ±16.48)μmol/L, P<0.01], while the ET-1 levels of the two groups with diabetes mellitus were significantly higher [(100.25 ±20.34) and (77.55 ±14.84) versus (53.62 ±8.40)ng/L, P<0.01] than those of the group C.The NO levels of group A were significantly lower than in group B [(43.87 ±12.05) versus (53.29 ±11.75)μmol/L, P<0.01].Moreover, the ET-1 levels of the group A were significantly higher than in group B [(100.25 ±20.34) versus (77.55 ±14.84)ng/L, P<0.01].Between the two diabetic groups, group A showed significantly higher systolic blood pressure(SBP), diastolic blood pressure(DBP), HbA1c, and course than group B (P<0.01).Correlation analysis showed a negative correlation between SBP, DBP, fasting blood glucose, HbA1c, and NO a positive correlation between high-density lipoprotein cholesterol ( HDL-C ) and NO, a negative correlation between HDL-C and ET-1, and a positive correlation between SBP,LDL-C, uric acid, fasting blood glucose, HbA1c, and ET-1.Conclusion The serum levels of NO and ET-1 in diabetic patients are evidently abnormal.Vascular endothelium injury will become more serious in patients with complications.Therefore, the serum levels of NO and ET-1 in diabetic patients are correlated with control of blood glucose, blood pressure, and blood lipid levels.%目的：探讨合并血管并发症的2型糖尿病患者血清一氧化氮（ nitric oxide，NO）和内皮素（ endothelin，ET）水平的变化，并分析各危险因素与两者之间的关系。方法入选2型糖尿病患者98例，根据分组条件分为糖尿病合并血管并发症组（ A组，49例）和糖尿病无血管并发症组（ B组，49例），同期选择年龄、体质指数相匹配的健康对照44例（ C组）。所有受试者记录身高、体质量、血压、病程，抽血检测空腹血糖（ fasting blood-glucose，FBG）、糖化血红蛋白（hemoglobin A1c，HbA1c）、血脂各项指标，以及血清NO和ET-1水平。结果与C组比较，A组及B组的NO水平均显著降低[（43．87±12．05）和（53．29±11．75）μmol／L vs （66．08±16．48）μmol／L，P＜0．01]，而ET-1水平显著升高[（100．25±20．34）和（77．55±14．84）ng／L vs （53．62±8．40）ng／L，P＜0．01]。 A组NO水平显著低于B组[（43．87±12．05） vs （53．29±11．75）μmol／L，P＜0．01]，而 ET-1水平显著高于B组[（100．25±20．34） vs （77．55±14．84）ng／L ，P＜0．01]。在糖尿病两组之间，收缩压、舒张压、HbA1c及病程，A组明显高于B组（P＜0．01）。相关分析显示，舒张压、收缩压、空腹血糖、HbA1c与NO之间存在负相关；HDL-C与NO之间存在正相关。HDL-C与ET-1存在负相关；收缩压、LDL-C、血尿酸、空腹血糖、HbA1c与ET-1之间存在正相关。结论糖尿病患者血清NO和ET水平明显异常，存在血管内皮功能损伤；有血管并发症者损伤更严重，且与血糖、血压、血脂的控制水平具有相关性。
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