2单位冰冻红细胞自动化洗涤程序研究

摘要

Objective To improve the processing protocol of the frozen erythrocyte ( RBC) washing machine of the dialysis type to enhance its efficiency of treating the 2 U frozen RBCs .Methods The frozen RBC washing machine for dialysis was used to deglycerolize the 2 U frozen RBCs after thawing .Measures including adjustment of the Pump speed , washing buffer adding and solution osmolarity were taken to decrease the processing time .Hemoglobin(Hb) content, Hb recovery, residual glycerol, free Hb content, deforming ability, and apoptosis of the deglycerolized RBCs were assessed to evaluate the efficiency of the improved protocol .Results As for the cells processed respectively by the previous and improved protocols, contents of Hb were (35.03 ±4.20) g and (53.82 ±2.08) g, respectively; Hb recoveries were (61.10 ±8.46)% and (80.22 ±3.73)%,respectively.Osmolarities representing residual glycerol were (307.00 ± 14.50)mOsm and (322.00 ±29.00)mOsm.Free Hb values were (0.74 ±0.04) and (0.80 ±0.08)g/L.The processing time was (1.64 ±0.31) and (1.04 ±0.16) h,respectively.There were significant differences in Hb content , Hb recovery, and deforming ability between the cells processed by the two protocols respectively .However , there were no significant changes in glycerol contents , free Hb, and apoptosis extent between the cells processed by the two protocols .Conclusion The innovated protocol can improve the quality of washed frozen RBCs , decrease the processing time and enhance the efficiency of the machine.%目的：对透析式冰冻红细胞（ RBC）洗涤机的处理程序进行改进，使之适用于2 U冰冻RBC的洗涤处理。方法深低温保存2 U冰冻RBC解冻复温后，采用透析式冰冻RBC洗涤机进行洗涤处理，对洗涤程序进行了提高泵速和加液速度、调整溶液渗透压等方面的改进，大幅缩短2 U冰冻RBC洗涤处理时间。对洗涤后的RBC进行血红蛋白（ Hb）含量、Hb回收率、甘油残留量（上清渗透压）、游离Hb含量、变形性以及凋亡率等指标的检测，并据此判断洗涤效果。结果改进前后的透析式洗涤程序处理得到的RBC的Hb含量分别为（35．03±4．20）和（53．82±2．08）g；Hb回收率分别为（61．10±8．46）％和（80．22±3．73）％；上清渗透压分别为（307．00±14．50）和（322．00± 29．00）mOsm；游离Hb分别为（0．74±0．04）和（0．80±0．08）g／L；程序改进前后的洗涤时间分别为（1．64±0．31）和（1．04±0．16）h；改进前后的洗涤程序处理得到的RBC在Hb含量、Hb回收率、RBC变形性、洗涤时间等指标上有明显改善。而在甘油残留量、游离Hb含量、细胞凋亡等方面无显著变化。结论经过程序改进，2 U冰冻RBC洗涤时间缩短、洗涤后RBC和Hb含量显著升高，提高了洗涤效率及冰冻RBC的利用效率。