不同抗氧化剂配伍组合对抗氧化能力的影响

摘要

目的：比较依达拉奉（edaravone）、还原型谷胱甘肽（GSH）和维生素C（VC）3种常用抗氧化剂进行配伍组合后抗氧化能力的变化情况，为后续复合抗氧化剂的筛选及临床应用提供参考依据。方法通过体外测定抗氧化剂的总抗氧化能力、还原能力、清除羟自由基能力和清除超氧阴离子能力，比较分析6组抗氧化剂的抗氧化活性强弱。结果与结论实验结果表明，在相同摩尔浓度下，复合抗氧化剂具有协同清除自由基的作用，其抗氧化活性得到显著提高。提示在研究抗氧化剂联合使用时，需要考虑到不同抗氧化剂联用的增效作用，可为后续临床应用选择合理的复合抗氧化剂提供实验依据。%Objective To provide reference for clinical application of combined antoxidants , by evaluating the com-bined and individual antioxidant activities of three commonly used antioxidants :edaravone,glutathion(GSH) and vitamin C ( VC) .Methods The multiple and individual antioxidant activities of the three antioxidants were compared by determining total antioxidant activity assay , reductive activity assay , superoxide anion scavenging assay and hydroxyl radical scavenging assay.Results and Conclusion Results showed that the combined antioxidants acted synergistically in scavenging free radicals and increased remarkably antioxidant activity at the same molar concentration .The rationality of different antioxi-dant combination should be taken into consideration in subsequent clinical application .