基于表面增强拉曼散射的血清指纹谱检测方法研究

摘要

Objective To optimize the experiment conditions of surface-enhanced Raman spectroscopy detection of serum fingerprint spectra.Methods Normal human serum was used as the sample and Ag nanoparticles as the active substrate.The enhanced signals of different optimized experiments were obtained , including serum dose(2.5 to 500 μl), incubation time(10 to 30 minutes) temperature(4℃,room temperature and 37℃),and different treatment(extraction and protein removal).Results and Conclusion Serum doses should not exceed 50μl.The ratio should range from 1∶1 to 5∶1, the incubation time is from 10 to 30 minutes, and the incubation temperature from 4℃ to 37℃.The signals of samples directly mixed with an active substrate are stronger than those of samples which are extracted or protein removed .%目的：对基于表面增强拉曼光谱检测血清指纹谱的试验条件进行优化。方法以正常人血清为例，银胶溶液为活性基底，分别检测不同血清用量（2．5～500μl）、不同孵育时间（10～30 min）、不同孵育温度（4℃、室温、37℃）及不同血清处理方法（萃取、去蛋白）的增强拉曼信号。结果及结论血清用量不宜超过50μl，与增强基底材料的比例1∶1到5∶1均适宜；孵育时间在10～30 min均可；孵育温度为4℃、室温、37℃均可；血清直接与增强基底混合信号效果极强，进行萃取和去蛋白处理后，拉曼信号会减弱。