[目的]以不同植物根及根际土壤为研究材料,进行植物根际促生菌(PGPR)的筛选,并探索其植物促生作用机制.[方法]以解磷、固氮、产氨、产IAA和拮抗3种常见病原真菌为筛选标准,测定了初筛菌株的多项促生能力,并通过对这些菌分别单独回接和多菌混接的玉米盆栽试验,测定了其对玉米的促生效应.[结果]从渭南、成阳、安康、商洛和榆林5地分离得到的158株菌中有17株茵具有上述多种植物促生作用的菌株.盆栽试验的测定结果表明:单独接种和多菌混合接种在玉米株高、根长、茎长、茎平均直径和干重方面与对照组相比较都有所增加,尤其是在多个指标上,多菌混合接种所显示出的促生效应均明显优于单菌接种.[结论]所筛选到的具有多种促生能力的菌株,可以为进一步构建植物根际促生菌(PGPR)菌群提供良好的种质资源.%[Objective] We used different root and rhizosphere soils in Weinan, Xianyang, Ankang, Shangluo and Yulin, Shaanxi province of China to isolate plant growth promoting rhizobacteria (PGPR), and then studied the mechanism why they can promote the growth of plants.[Methods] Preliminary screening of PGPRs under the premises of PGPR may having the abilities of phosphate solubilization, N2-fixing, production of NH3 and indoleacetic acid (IAA) and antagonistic activity against three common pathogenic fungi.After that, multiple plant growth promoting activities were detected, maize growth enhancement by plant inoculation studies with these isolates alone individually and the mixture of each isolates under pot experiment conditions.[Results] In total of 158 strains were isolated.17 of which have mechanisms and a striking plant growth promoting activity with respect to various plant parameters.Underpotcultureconditions,compared with the control, the results showed that the strain which inoculated in combination was significant increase than inoculated alone, which in shoot length, root length, stem length, average diameter of stem and plant dry weight, respectively.[Conclusion] Isolates with multiple PGP activities can also be rhizospheric competent, providing promising isolates for PGPRs combination to resolve the challenges in field application of PGPR.
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