Objective To observe the expression of helper T cell 1 ( Th1) ,helper T cell 2 ( Th2) and helper T cell 17 ( Th17) in serum of elderly patients with eczema,and to explore the correlation between serum cytokine levels and patho-logical characteristics of eczema. Methods The clinical data of 60 elderly patients diagnosed with eczema in our depart-ment between June 2013 and September 2015 were collected and 40 cases of healthy persons were selected as control group. The expression levels of serum Th cell related cytokines[ interferon-γ( INF-γ) ,interleukin-4 ( IL-4) ,interleukin-17A ( IL-17A)] were detected by enzyme linked immunosorbent assay (ELISA).The differences of Th cell related cytokines in acute and chronic,or generalized and localized eczema were compared. Results The levels of serum IFN-γ,IL-4 and IL-17A in elderly patients with eczema were significantly higher than those in the healthy control group ( t=10.570,10. 874,14.611;all P<0.001).The levels of serum IFN-γ,IL-4 and IL-17A in elderly patients with acute eczema were significantly higher than those in patients with chronic eczema ( t=3.787,3.815,5.338,all P<0.001);The levels of serum IFN-γ,IL-4 and IL-17A in patients with generalized eczema were significantly higher than those in patients with localized eczema ( t=2.458,2. 139,3.019;all P<0.05). Conclusion Serum IFN-γ,IL-4 and IL-17A are involved in the progression and prognosis of eczema,and the different expression of Th cytokines can be used as indicators for reflecting clinical types.%目的：观察辅助性T细胞1( Th1)、辅助性T细胞2( Th2)、辅助性T细胞17( Th17)在老年湿疹患者血清中的表达,探讨血清细胞因子水平与湿疹的病理特征的相关性。方法收集在本科确诊为老年湿疹患者60例的临床资料,并选取健康对照组40例。采用酶联免疫吸附法( ELISA)检测血清中Th的特征细胞因子γ-干扰素( INF-γ)、白介素-4( IL-4)及白介素-17A( IL-17A)的表达水平,分别比较Th细胞相关因子在急慢性,或泛发和局限型湿疹中的差异。结果与健康对照组相比,老年湿疹患者血清IFN-γ、IL-4、IL-17A显著升高(t＝10.570,10.874,14.611；均P<0.001)。其中老年急性湿疹患者血清IFN-γ、IL-4及IL-17A显著高于慢性期(t＝3.787,3.815,5.338；均P<0.001)；泛发型患者血清IFN-γ、IL-4及IL-17A明显高于局限型( t＝2.458,2.139,3.019；均P<0.05)。结论血清IFN-γ、IL-4及IL-17A参与老年湿疹的进展与预后,并且Th细胞相关因子表达的不同可作为反映临床类型的指标。
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