慢性间断性低氧大鼠脑皮层GAP-43表达的时间变化

摘要

Objective To observe the time variation of the growth associated protein on the cerebral cortex in chronic intermittent hypoxia rats for discussing the relation between the change and neural repair after hypoxic brain damage.Methods Forty adult male Sprague-Dawley rats were randomly averagely divided into four groups:control group,CIH 1 week group,CIH 3 weeks group and CIH 5 weeks group.CIH-handled rats were exposed to intermittent hypoxia in designed cabin 8 hours every day,and the duration of hypoxia was 1 weeks,3 weeks,5 weeks.The GAP-43 expression of hippocampus were detected by immunohistochemical staining.Results Compared with the control group,the GAP-43expression in cerebral cortex was increased in CIH rats.The peak of GAP-43 expression appeared at 1 week(P＜0.05)and then it reduced at 3 weeks(P＜0.05)and it was closed to the level of control group at 5 weeks(P＞0.05).Conclusion There was the time variation of the growth associated protein expression on the cerebral cortex in chronic intermittent hypoxia rats,which maybe associated with the compensatory repair to against chronic intermittent hypoxia.%目的 观察慢性间断性低氧(CIH)大鼠脑皮层生长相关蛋白(GAP-43)表达的时间变化,探讨这些变化与缺氧性脑损伤修复间的关系.方法 成年雄性SD大鼠40只,随机均分为对照组、CIH 1周、CIH 3周和CIH 5周组.CIH组大鼠在自制缺氧舱内每天缺氧8小时,连续缺氧1、3和5周.利用免疫组化方法检测大脑皮层神经元生长相关蛋白的表达.结果 与对照组比较,慢性间断性低氧大鼠脑皮层神经元GAP-43阳性表达增多,表达高峰出现在1周后(P＜0.05),3周后表达逐渐减少(P＞0.05),5周后表达接近对照组水平(P＞0.05).结论 慢性间断性低氧大鼠脑皮层生长相关蛋白的表达呈现时间变化,这可能与缺氧性脑损伤后的修复有关.