反义端粒酶RNA抑制胶质瘤细胞系BT－325细胞端粒酶活性表达

摘要

Objective:To observe the effect of antisense telomerase RNA on BT－325 cells and to investigate the role of antisense telomerase RNA in astrocytoma treatment.Methods:Antihygromycin antisense telomerase RNA eucaryotic expression vector,pBBS 212／hTR,was introduced into human astrocytoma cell lines BT-325 by using lipofectin mediation.TUNEL,TRAP-PAGE and electron microscope were used to simultaneously determine BT-325／pBBS 212-hTR apoptosis and telomerase activity after hygromycin screening and cloning.Results:Compared with control,cell apoptosis rose significantly in the course of antisense telomerase RNA inhibtion of BT-325 telomerase activity.Conclusion:Antisense telomerase RNA can promote BT-325 apoptosis by blocking telomerase activity.This is valuable to study astrocytoma genesis and to find the cure.%目的：观察反义端粒酶RNA对BT－325细胞的作用，探讨端粒酶在恶性胶质瘤发生过程中的可能作用及反义端粒酶RNA在恶性胶质瘤基因治疗中的意义。方法：经脂质体介导的方法将pBBS 212－hTR(反义端粒酶RNA真核表达载体)导入BT－325细胞中，细胞克隆转移扩大培养后，采用TRAP－PAGE电泳、电镜和TUNEL技术检测BT－325／pBBS 212－hTR细胞的端粒酶活性表达、细胞凋亡的情况。结果：与对照组比较，反义端粒酶RNA显著抑制BT－325细胞端粒酶活性、诱发细胞发生凋亡。结论：转染反义端粒酶RNA在封闭细胞端粒酶活性表达的同时，可促进BT－325细胞凋亡。在研究恶性胶质瘤发病机制及治疗方面做了有益的探讨。